What does immunoassay interference mean?

Published by Charlie Davidson on

What does immunoassay interference mean?

Substances that alter the measurable concentration of the analyte or alter antibody binding can potentially result in immunoassay interference. Interference in immunoassay may lead to the misinterpretation of a patient’s results by the laboratory and the wrong course of treatment being given by the physician.

How to detect and solve immunoassay interference?

The best way to resolve prozone interference is to dilute the samples and rerun the assay. Elevated endogenous serum components, such as bilirubin (icterus), hemoglobin (hemolysis), lipids (turbidity), and proteins interfere more in homogeneous than heterogeneous assays.

What is immunoassay interference drug test?

Abstract. Urine drug screen (UDS) immunoassays are a quick and inexpensive method for determining the presence of drugs of abuse. Many cross-reactivities exist with other analytes, potentially causing a false-positive result in an initial drug screen.

What are endogenous antibodies?

The 3 types of endogenous antibodies known to cause interferences in immunoassays are heterophile, antianimal, and autoantibodies. Heterophile antibodies are produced without exposure to specific immunogens and are thus considered to be naturally occurring.

What is immunoassay used for?

Immunoassays are used to quantify molecules of biological interest based on the specificity and selectivity of antibody reagents generated. In HTS and lead optimization projects, assays are designed to detect molecules that are produced intracellularly or secreted in response to compounds screened.

What does a positive heterophile antibody test mean?

A positive test means heterophile antibodies are present. These are most often a sign of mononucleosis. Your provider will also consider other blood test results and your symptoms. A small number of people with mononucleosis may never have a positive test.

How is an immunoassay test performed?

When immunoassays are used to test for the presence of an antibody in a blood or fluid sample, the test contains the specific antigen as part of the detection system. If the antibody being tested for is present in the sample, it will react with or bind to the antigen in the test system and will be detected as positive.

What are the factors involved in developing an immunoassay?

Important Parameters for Development of an Immunoassay

  • Capture antibody.
  • Detection antibody.
  • Plate type.
  • Coating buffer.
  • Blocking buffer/diluent buffer.
  • Wash buffer.
  • Coating antibody concentration.
  • Coated antibody stability.

What will cause a false positive for barbiturates?

Pain or Anti-Inflammatory Medication Ibuprofen, like Motrin or Advil, could lead to false positives for barbiturates, PCP, or THC. Aleve, or naproxen, may also lead to a false positive for barbiturates of THC.

Why is it called endogenous?

Processes caused by forces from within the Earth are endogenous processes. Exo is a prefix meaning “out”, and endo is a prefix meaning “in”. Many exogenous (extraterrestrial) forces are caused by other bodies in the Solar System.

What is an endogenous source?

Endogenous sources include errors of metabolism that lead to large accumulations of elements such as copper or iron, which are normally only found in trace quantities.

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