What is Eosin Azure?

Eosin Azure (EA) 50 is a counterstain used for polychromatic cytological staining of gynecological samples. Papanicolaou stain comprises of both the basic and acidic dyes. Basic components of the dye stain the acidic constituents of the cell while the acidic part stains the basic components of the cell.

What is EA 36?

Eosin Azure (EA 36), also known as Papanicolaou stain, is a counterstain used to differentiate cells in various bodily secretions. Unstained cells are practically invisible under a microscope; therefore, the cells are stained for easier interpretation of cytomorphological patterns.

What does EA stain?

The polychromatic EA solution stains the unstained cellular components, such as nucleoli, squamous cells, cilia, and erythrocytes. The test samples for Papanicolaou staining could be gynecological or non-gynecological including sputum, urine, and cytological samples.

Is Azure B Basic?

These are considered neutral stains because they are composed of a basic dye and an acidic dye; the dyes impart color to cellular components they are attracted to. Wright’s stain is a commonly used dye for staining blood smears. The basic dye in Wright’s stain is azure B and the acidic dye is eosin Y.

Which stain is used for Pap smear?

Papanicolaou stain (also Papanicolaou’s stain and Pap stain) is a multichromatic (multicolored) cytological staining technique developed by George Papanicolaou in 1942. The Papanicolaou stain is one of the most widely used stains in cytology, where it is used to aid pathologists in making a diagnosis.

What does Orange G stain?

Orange G is an acidic dye. It stains keratin a bright, intense orange. The granules in eosinophilic, superficial cells (possibly those containing eleidin) are also stained. The staining time must be limited or the subsequent uptake of eosin Y is inhibited.

How do you prepare og 6?

1) Rinse in 70% alcohol, 50% alcohol and distilled water. 2) Stain in Harris haematoxylin (without acetic acid) for 5 -10 minutes. 3) Rinse in distilled water. 4) Rinse 3 or 4 times in 0.5% aqueous solution of hydrochloric acid.

What color does keratin stain?

Cell cytoplasm stains blue-green and keratin stains orange in color. Eosin Y stains the superficial epithelial squamous cells, nucleoli, cilia, and red blood cells.

What is the most critical step in the PAP staining?

As a limitation of this study, we should mention that UF-PAP stain is very sensitive technique, thus air-drying is a critical step. Also, due to the omission of O-G-6 in UF-PAP method, this method cannot be used for the diagnosis of squamous cell carcinoma.

What is eosin used for?

Eosin can be used to stain cytoplasm, red blood cells, collagen, and muscle fibers for histological examination. It is most often used as a counterstain to hematoxylin in H&E staining. In H&E, eosin Y is typically used in concentrations of 0.5–1% (0.5–1 g eosin Y in 100 ml distilled water or 75% ethanol).

What is stained smear?

The purpose of making a smear is to fix the bacteria onto the slide and to prevent the sample from being lost during a staining procedure. A smear can be prepared from a solid or broth medium. Below are some guidelines for preparing a smear for a Gram-stain.

Is H&E staining better than PAP staining?

We conclude that the efficacy of distinct identification of KP and ICK in oral SCC by modified PAP stain is better than H&E stain. But H&E is gold standard and simple stain in demonstrating other details like nucleus, connective tissue structures.

Which is the second counterstain for eosin Azure?

Eosin Azure : This is the second counterstain which is a polychrome mixture of eosin Y, light green SF and Bismarck brown. Eosin Y gives a pink colour to cytoplasm of mature squamous cells, nucleoli, cilia and red blood cells. Staining solutions commonly used in cytology are EA 31 and EA 50, while EA 65

What kind of stain is Azure eosin methylene blue?

Compare Tool. Synonyms: Azure eosin methylene blue; Giemsa solution CAS No.: 51811-82-6 Principle: When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration.

What are the synonyms for eosin azure blue?

Synonyms: Azure eosin methylene blue; Giemsa solution CAS Number: 51811-82-6 MDL No.: MFCD00081642 EC Number: 200-659-6 UNSPSC Code: 12352200 Application: Tested for staining blood smears according to G. Clark (ed.), Staining Procedures, 4th edition, Williams & Wilkins, 173 (1981);…

What is the Colour Index of azure B?

The stock solution includes azure B, tetrafluoroborate or thiocyanate (Colour Index 52010), > 80% pure, and eosin Y (Colour Index 45380), > 80% pure. Dissolve 0.6 g of azure B in 60 ml of dimethyl sulphoxide (DMSO) and 0.2 g of eosin Y in 50 ml of DMSO; preheat the DMSO to 37 °C before adding the dyes.