What does rotenone do to the mitochondria?

Published by Charlie Davidson on

What does rotenone do to the mitochondria?

Rotenone acts as a strong inhibitor of the mitochondrial complex I. The resulting incomplete electron transfer within the MRC leads to ATP depletion and in turn promotes the formation of ROS and thereby induces oxidative stress and apoptosis in cells2,3.

What is mitochondrial morphology?

Mitochondrial morphology encompasses a vast spectrum from small spheres/ovals and short tubules to elongated tubules and reticular networks depending on cell types in varied tissues and organs. Among many types of mitochondrial shape changes, fission and fusion are the most defined and studied processes.

Does rotenone inhibit Complex 2?

It inhibits the transfer of electrons from iron-sulfur centers in CI to ubiquinone via binding to the ubiquinone binding site of complex I [2]. However, this compound appears to exert any effects on ion currents in different types of cells.

How does rotenone lead to cell death?

Rotenone induces cell death in primary dopaminergic culture by increasing ROS production and inhibiting mitochondrial respiration. Neurochem Int. 2006 Sep;49(4):379-86. doi: 10.1016/j.

Is rotenone an Uncoupler?

Rotenone is used in biomedical research to study oxygen consumption rate of cells usually in combination with antimycin A (an electron transport chain Complex III inhibitor), oligomycin (an ATP synthase inhibitor) and FCCP (a mitochondrial uncoupler).

Can you eat fish killed by rotenone?

Can we eat fish treated by rotenone? You are strongly cautioned not to eat fish that have been treated with rotenone because no federal or state guidelines are in place for eating fish taken after rotenone treatment. If they were, the un-neutralized rotenone would kill them.

What are 4 structures of the mitochondria?

Structure

  • The outer mitochondrial membrane,
  • The intermembrane space (the space between the outer and inner membranes),
  • The inner mitochondrial membrane,
  • The cristae space (formed by infoldings of the inner membrane), and.
  • The matrix (space within the inner membrane),which is a fluid.

Is rotenone still used?

Rotenone’s use as a pesticide has already been discontinued in the US as of 2005 due to health concerns***, but shockingly, it’s still poured into our waters every year by fisheries management officials as a piscicide to remove unwanted fish species.

Why is rotenone toxic to fish?

Rotenone kills fish by inhibiting cellular respiration and the ability to use dis- solved oxygen. In effect, the fish suffocate. Fish exposed to lethal rotenone concentrations move to the surface and gasp for oxygen as if the water was oxygen-depleted.

Do mitochondria have 2 membranes?

As previously mentioned, mitochondria contain two major membranes. The outer mitochondrial membrane fully surrounds the inner membrane, with a small intermembrane space in between. The inner membrane is also loaded with proteins involved in electron transport and ATP synthesis.

Where can mitochondria be found?

Mitochondria are structures within cells that convert the energy from food into a form that cells can use. Each cell contains hundreds to thousands of mitochondria, which are located in the fluid that surrounds the nucleus (the cytoplasm).

How is rotenone used to treat mitochondrial Complex I?

Rotenone was able to induce mitochondrial complex I substrate-supported mitochondrial ROS production both in isolated mitochondria from HL-60 cells as well as in cultured cells. Rotenone-induced apoptosis was confirmed by DNA fragmentation, cytochrome c release, and caspase 3 activity.

How is rotenone used to induce apoptosis?

These results suggest that rotenone is able to induce apoptosis via enhancing the amount of mitochondrial reactive oxygen species production. MeSH terms Apoptosis / drug effects*

How does rotenone inhibit microtubule assembly and proliferation?

In addition to the effects on the MRC, several studies have demonstrated that rotenone inhibits microtubule assembly independently of a specific energy-requiring step through tubulin binding, resulting in mitotic arrest and inhibition of cell proliferation 5, 6, 7.

What kind of degeneration is caused by rotenone?

Chronic administration of rotenone has caused selective degeneration of nigral dopaminergic neurons with histopathological hallmarks of PD and PD-like locomotor symptoms in animal models 14.

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